*Target (protein/gene name): DIHYDROLIPOAMIDE DEHYDROGENASE LPD (LIPOAMIDE REDUCTASE (NADH)) (LIPOYL DEHYDROGENASE) (DIHYDROLIPOYL DEHYDROGENASE) (DIAPHORASE *NCBI Gene # or RefSeq#:
75766096 *Protein ID (NP or XP #) or Wolbachia#:
Rv0462 *Organism (including strain): Mycobacterium tuberculosis H37Rv
Etiologic Risk Group (see link below):
Risk Group 3
*Background/Disease Information:
Tuberculosis is a serious infectious disease affecting the lungs, and it can be fatal if untreated. The disease is caused by the bacterium Mycobacterium tuberculosis, which is released into the air when an infected person coughs or sneezes, making the disease highly contagious. Symptoms of tuberculosis include chronic cough, chest pain, coughing up blood or septum, night sweats, fever, and weight loss. People can become infected with the Mycobacterium tuberculosis without developing the disease because it can remain latent in the body. Certain individuals are more susceptible to tuberculosis, such as people infected with HIV. Because of the growing problem of bacterial resistance, it is important that new methods for treating tuberculosis and other bacterial diseases are developed.
Essentiality of this protein: Gene/Ortholog: Tb11.01.8470 (OG4_10422); Phenotype: significant gain of fitness in bloodstream forms (3 days); Source study: alsford Gene/Ortholog: Tb11.01.8470 (OG4_10422); Phenotype: significant gain of fitness in bloodstream forms (6 days); Source study: alsford Gene/Ortholog: Tb11.01.8470 (OG4_10422); Phenotype: significant loss of fitness in procyclic forms; Source study: alsford Gene/Ortholog: Tb11.01.8470 (OG4_10422); Phenotype: significant loss of fitness in differentiation of procyclic to bloodstream forms; Source study: alsford
Complex of proteins?:
No
Druggable Target (list number or cite evidence from a paper/database showing druggable in another organism):
Druggabilty: 0.5
Image of protein (PyMol with features delineated and shown separately): *Amino Acid Sequence (paste as text only - not as screenshot or as 'code'):
mthydvvvlg agpggyvaai raaqlglsta ivepkywggv clnvgcipsk allrnaelvh
iftkdakafg isgevtfdyg iaydrsrkva egrvagvhfl mkknkiteih gygtfadant
llvdlndggt esvtfdnaii atgsstrlvp gtslsanvvt yeeqilsrel pksiiiagag
aigmefgyvl knygvdvtiv eflpralpne dadvskeiek qfkklgvtil tatkvesiad
ggsqvtvtvt kdgvaqelka ekvlqaigfa pnvegygldk agvaltdrka igvddymrtn
vghiyaigdv ngllqlahva eaqgvvaaet iagaetltlg dhrmlpratf cqpnvasfgl
teqqarnegy dvvvakfpft anakahgvgd psgfvklvad akhgellggh lvghdvaell
peltlaqrwd ltaselarnv hthptmseal qecfhglvgh minf
*length of your protein in Amino Acids: 465 aa
Molecular Weight of your protein in kiloDaltons using the Expasy ProtParam website
49239.1 g/mol
Molar Extinction coefficient of your protein at 280 nm wavelength:
Ext. coefficient 30620
Abs 0.1% (=1 g/l) 0.622, assuming all pairs of Cys residues form cystines
Ext. coefficient 30370
Abs 0.1% (=1 g/l) 0.617, assuming all Cys residues are reduced
*GC% Content for gene: *CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol (paste as text only): *GC% Content for gene (codon optimized)
DIHYDROLIPOAMIDE DEHYDROGENASE LPD (LIPOAMIDE REDUCTASE (NADH)) (LIPOYL DEHYDROGENASE) (DIHYDROLIPOYL DEHYDROGENASE) (DIAPHORASE
*NCBI Gene # or RefSeq#:
75766096
*Protein ID (NP or XP #) or Wolbachia#:
Rv0462
*Organism (including strain):
Mycobacterium tuberculosis H37Rv
Etiologic Risk Group (see link below):
Risk Group 3
*Background/Disease Information:
Tuberculosis is a serious infectious disease affecting the lungs, and it can be fatal if untreated. The disease is caused by the bacterium Mycobacterium tuberculosis, which is released into the air when an infected person coughs or sneezes, making the disease highly contagious. Symptoms of tuberculosis include chronic cough, chest pain, coughing up blood or septum, night sweats, fever, and weight loss. People can become infected with the Mycobacterium tuberculosis without developing the disease because it can remain latent in the body. Certain individuals are more susceptible to tuberculosis, such as people infected with HIV. Because of the growing problem of bacterial resistance, it is important that new methods for treating tuberculosis and other bacterial diseases are developed.
Link to TDR Targets page (if present):
http://www.tdrtargets.org/targets/view?gene_id=6322
Link to Gene Database page (NCBI, EuPath databases -e.g. TryTryp, PlasmoDB, etc - or PATRIC, etc.)
http://www.ncbi.nlm.nih.gov/protein/?term=2A8X
Essentiality of this protein:
Gene/Ortholog: Tb11.01.8470 (OG4_10422); Phenotype: significant gain of fitness in bloodstream forms (3 days); Source study: alsford
Gene/Ortholog: Tb11.01.8470 (OG4_10422); Phenotype: significant gain of fitness in bloodstream forms (6 days); Source study: alsford
Gene/Ortholog: Tb11.01.8470 (OG4_10422); Phenotype: significant loss of fitness in procyclic forms; Source study: alsford
Gene/Ortholog: Tb11.01.8470 (OG4_10422); Phenotype: significant loss of fitness in differentiation of procyclic to bloodstream forms; Source study: alsford
Complex of proteins?:
No
Druggable Target (list number or cite evidence from a paper/database showing druggable in another organism):
Druggabilty: 0.5
*EC#:
1.8.1.4
Link to BRENDA EC# page:
http://www.brenda-enzymes.org/php/result_flat.php4?ecno=1.8.1.4
Enzyme Assay information (spectrophotometric, coupled assay ?, reagents):
-- link to Sigma (or other company) page for assay (see Sigma links below)
http://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Enzyme_Assay/lipoamidedehydrogenase.pdf
-- links to assay reagents (substrates) pages.
--- List cost and quantity of substrate reagents, supplier, and catalog #
A. 50 mM Sodium Phosphate Buffer, pH 6.5 at 25°C
Sigma Prod. No. S-0751
B. 28 mM DL-Thioctic Acid Amide Solution (DL-Thio)
Sigma Prod. No. T-5875
C. 7.0 mM ß-Nicotinamide Adenine Dinucleotide, Reduced Form Solution (ß-NADH)
Sigma Prod.No. N-8129
Sigma Stock No. 340-110
D. 20 mM ß-Nicotinamide Adenine Dinucleotide, Oxidized Form Solution (ß-NAD)
Sigma Stock No. 260-150
Sigma Prod. No. N-7004
E. 300 mM Ethylenediaminetetraacetic Acid with 2.0% (w/v) Albumin Solution, pH 7.0 at 25°C (EDTA)
Sigma Stock No. ED4S
Sigma Prod. No. A-4503
F. Lipoamide Dehydrogenase Enzyme Solution
Structure Available (PDB or Homology model)
2A8X
3II4
Current Inhibitors:
CHEMBL405110
Expression Information (has it been expressed in bacterial cells):
The protein has been expressed in E. coli.
http://www.ncbi.nlm.nih.gov/pubmed/11560483
Purification Method:
http://pubs.acs.org/doi/pdf/10.1021/bi010575o
Image of protein (PyMol with features delineated and shown separately):
*Amino Acid Sequence (paste as text only - not as screenshot or as 'code'):
mthydvvvlg agpggyvaai raaqlglsta ivepkywggv clnvgcipsk allrnaelvh
iftkdakafg isgevtfdyg iaydrsrkva egrvagvhfl mkknkiteih gygtfadant
llvdlndggt esvtfdnaii atgsstrlvp gtslsanvvt yeeqilsrel pksiiiagag
aigmefgyvl knygvdvtiv eflpralpne dadvskeiek qfkklgvtil tatkvesiad
ggsqvtvtvt kdgvaqelka ekvlqaigfa pnvegygldk agvaltdrka igvddymrtn
vghiyaigdv ngllqlahva eaqgvvaaet iagaetltlg dhrmlpratf cqpnvasfgl
teqqarnegy dvvvakfpft anakahgvgd psgfvklvad akhgellggh lvghdvaell
peltlaqrwd ltaselarnv hthptmseal qecfhglvgh minf
*length of your protein in Amino Acids: 465 aa
Molecular Weight of your protein in kiloDaltons using the Expasy ProtParam website
49239.1 g/mol
Molar Extinction coefficient of your protein at 280 nm wavelength:
Ext. coefficient 30620
Abs 0.1% (=1 g/l) 0.622, assuming all pairs of Cys residues form cystines
Ext. coefficient 30370
Abs 0.1% (=1 g/l) 0.617, assuming all Cys residues are reduced
TMpred graph Image (http://www.ch.embnet.org/software/TMPRED_form.html). Input your amino acid sequence to it.
CDS Gene Sequence (paste as text only):
gtgacccact atgacgtcgt cgttctcgga gccggtcccg gcgggtatgt cgcggcgatt
cgcgccgcac agctcggcct gagcactgca atcgtcgaac ccaagtactg gggcggagta
tgcctcaatg tcggctgtat cccatccaag gcgctgttgc gcaacgccga actggtccac
atcttcacca aggacgccaa agcatttggc atcagcggcg aggtgacctt cgactacggc
atcgcctatg accgcagccg aaaggtagcc gagggcaggg tggccggtgt gcacttcctg
atgaagaaga acaagatcac cgagatccac gggtacggca catttgccga cgccaacacg
ttgttggttg atctcaacga cggcggtaca gaatcggtca cgttcgacaa cgccatcatc
gcgaccggca gtagcacccg gctggttccc ggcacctcac tgtcggccaa cgtagtcacc
tacgaggaac agatcctgtc ccgagagctg ccgaaatcga tcattattgc cggagctggt
gccattggca tggagttcgg ctacgtgctg aagaactacg gcgttgacgt gaccatcgtg
gaattccttc cgcgggcgct gcccaacgag gacgccgatg tgtccaagga gatcgagaag
cagttcaaaa agctgggtgt cacgatcctg accgccacga aggtcgagtc catcgccgat
ggcgggtcgc aggtcaccgt gaccgtcacc aaggacggcg tggcgcaaga gcttaaggcg
gaaaaggtgt tgcaggccat cggatttgcg cccaacgtcg aagggtacgg gctggacaag
gcaggcgtcg cgctgaccga ccgcaaggct atcggtgtcg acgactacat gcgtaccaac
gtgggccaca tctacgctat cggcgatgtc aatggattac tgcagctggc gcacgtcgcc
gaggcacaag gcgtggtagc cgccgaaacc attgccggtg cagagacttt gacgctgggc
gaccatcgga tgttgccgcg cgcgacgttc tgtcagccaa acgttgccag cttcgggctc
accgagcagc aagcccgcaa cgaaggttac gacgtggtgg tggccaagtt cccgttcacg
gccaacgcca aggcgcacgg cgtgggtgac cccagtgggt tcgtcaagct ggtggccgac
gccaagcacg gcgagctact gggtgggcac ctggtcggcc acgacgtggc cgagctgctg
ccggagctca cgctggcgca gaggtgggac ctgaccgcca gcgagctggc tcgcaacgtc
cacacccacc caacgatgtc tgaggcgctg caggagtgct tccacggcct ggttggccac
atgatcaatt tctga
*GC% Content for gene:
*CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol (paste as text only):
*GC% Content for gene (codon optimized)